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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB100-56593 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56593, RRID:AB_839049
- Product name
- Mouse Monoclonal TRANCE/TNFSF11/RANK L Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references RANKL-mediated osteoclastogenic differentiation of macrophages in the abdominal aorta of angiotensin II-infused apolipoprotein E knockout mice.
Multiplexed quantum dot labeling of activated c-Met signaling in castration-resistant human prostate cancer.
The inhibition of RANKL/RANK signaling by osteoprotegerin suppresses bone invasion by oral squamous cell carcinoma cells.
Expression of tumor invasion factors determines systemic engraftment and induction of humoral hypercalcemia in a mouse model of adult T-cell leukemia.
Expansion of antigen-specific regulatory T cells with the topical vitamin d analog calcipotriol.
Ameloblastoma induces osteoclastogenesis: a possible role of ameloblastoma in expanding in the bone.
Prostate carcinoma cells that have resided in bone have an upregulated IGF-I axis.
Multiple myeloma disrupts the TRANCE/ osteoprotegerin cytokine axis to trigger bone destruction and promote tumor progression.
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines.
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines.
Gene expression of osteoclast differentiation factor is induced by lipopolysaccharide in mouse osteoblasts via Toll-like receptors.
Gene expression of osteoclast differentiation factor is induced by lipopolysaccharide in mouse osteoblasts via Toll-like receptors.
Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ligand.
Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ligand.
Tanaka T, Kelly M, Takei Y, Yamanouchi D
Journal of vascular surgery 2018 Dec;68(6S):48S-59S.e1
Journal of vascular surgery 2018 Dec;68(6S):48S-59S.e1
Multiplexed quantum dot labeling of activated c-Met signaling in castration-resistant human prostate cancer.
Hu P, Chu GC, Zhu G, Yang H, Luthringer D, Prins G, Habib F, Wang Y, Wang R, Chung LW, Zhau HE
PloS one 2011;6(12):e28670
PloS one 2011;6(12):e28670
The inhibition of RANKL/RANK signaling by osteoprotegerin suppresses bone invasion by oral squamous cell carcinoma cells.
Shin M, Matsuo K, Tada T, Fukushima H, Furuta H, Ozeki S, Kadowaki T, Yamamoto K, Okamoto M, Jimi E
Carcinogenesis 2011 Nov;32(11):1634-40
Carcinogenesis 2011 Nov;32(11):1634-40
Expression of tumor invasion factors determines systemic engraftment and induction of humoral hypercalcemia in a mouse model of adult T-cell leukemia.
Parrula C, Zimmerman B, Nadella P, Shu S, Rosol T, Fernandez S, Lairmore M, Niewiesk S
Veterinary pathology 2009 Sep;46(5):1003-14
Veterinary pathology 2009 Sep;46(5):1003-14
Expansion of antigen-specific regulatory T cells with the topical vitamin d analog calcipotriol.
Ghoreishi M, Bach P, Obst J, Komba M, Fleet JC, Dutz JP
Journal of immunology (Baltimore, Md. : 1950) 2009 May 15;182(10):6071-8
Journal of immunology (Baltimore, Md. : 1950) 2009 May 15;182(10):6071-8
Ameloblastoma induces osteoclastogenesis: a possible role of ameloblastoma in expanding in the bone.
Sandra F, Hendarmin L, Kukita T, Nakao Y, Nakamura N, Nakamura S
Oral oncology 2005 Jul;41(6):637-44
Oral oncology 2005 Jul;41(6):637-44
Prostate carcinoma cells that have resided in bone have an upregulated IGF-I axis.
Rubin J, Chung LW, Fan X, Zhu L, Murphy TC, Nanes MS, Rosen CJ
The Prostate 2004 Jan 1;58(1):41-9
The Prostate 2004 Jan 1;58(1):41-9
Multiple myeloma disrupts the TRANCE/ osteoprotegerin cytokine axis to trigger bone destruction and promote tumor progression.
Pearse RN, Sordillo EM, Yaccoby S, Wong BR, Liau DF, Colman N, Michaeli J, Epstein J, Choi Y
Proceedings of the National Academy of Sciences of the United States of America 2001 Sep 25;98(20):11581-6
Proceedings of the National Academy of Sciences of the United States of America 2001 Sep 25;98(20):11581-6
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines.
Fiumara P, Snell V, Li Y, Mukhopadhyay A, Younes M, Gillenwater AM, Cabanillas F, Aggarwal BB, Younes A
Blood 2001 Nov 1;98(9):2784-90
Blood 2001 Nov 1;98(9):2784-90
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines.
Fiumara P, Snell V, Li Y, Mukhopadhyay A, Younes M, Gillenwater AM, Cabanillas F, Aggarwal BB, Younes A
Blood 2001 Nov 1;98(9):2784-90
Blood 2001 Nov 1;98(9):2784-90
Gene expression of osteoclast differentiation factor is induced by lipopolysaccharide in mouse osteoblasts via Toll-like receptors.
Kikuchi T, Matsuguchi T, Tsuboi N, Mitani A, Tanaka S, Matsuoka M, Yamamoto G, Hishikawa T, Noguchi T, Yoshikai Y
Journal of immunology (Baltimore, Md. : 1950) 2001 Mar 1;166(5):3574-9
Journal of immunology (Baltimore, Md. : 1950) 2001 Mar 1;166(5):3574-9
Gene expression of osteoclast differentiation factor is induced by lipopolysaccharide in mouse osteoblasts via Toll-like receptors.
Kikuchi T, Matsuguchi T, Tsuboi N, Mitani A, Tanaka S, Matsuoka M, Yamamoto G, Hishikawa T, Noguchi T, Yoshikai Y
Journal of immunology (Baltimore, Md. : 1950) 2001 Mar 1;166(5):3574-9
Journal of immunology (Baltimore, Md. : 1950) 2001 Mar 1;166(5):3574-9
Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ligand.
Hsu H, Lacey DL, Dunstan CR, Solovyev I, Colombero A, Timms E, Tan HL, Elliott G, Kelley MJ, Sarosi I, Wang L, Xia XZ, Elliott R, Chiu L, Black T, Scully S, Capparelli C, Morony S, Shimamoto G, Bass MB, Boyle WJ
Proceedings of the National Academy of Sciences of the United States of America 1999 Mar 30;96(7):3540-5
Proceedings of the National Academy of Sciences of the United States of America 1999 Mar 30;96(7):3540-5
Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ligand.
Hsu H, Lacey DL, Dunstan CR, Solovyev I, Colombero A, Timms E, Tan HL, Elliott G, Kelley MJ, Sarosi I, Wang L, Xia XZ, Elliott R, Chiu L, Black T, Scully S, Capparelli C, Morony S, Shimamoto G, Bass MB, Boyle WJ
Proceedings of the National Academy of Sciences of the United States of America 1999 Mar 30;96(7):3540-5
Proceedings of the National Academy of Sciences of the United States of America 1999 Mar 30;96(7):3540-5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Analysis of human lymph node lysate (35ug per lane, RIPA buffer) at 0.5ug/ml. Band detected at ~35kDa and ~28kDa. (Expected MW of 35.5kDa according to NP_003692.1 and of 27.7kDa according to NP_143026.1)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Analysis of transfected cell lysate was probed with 2 ug of Trance antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Formalin-fixed, paraffin-embedded human lymph node probed with Trance antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A668) - Azide Free [NB100-56593] - Formalin-fixed, paraffin-embedded human liver stained with Trance antibody (1:500, 1 ug/ml), peroxidase-conjugate and DAB chromogen. A 2 hr incubation at RT was used.
