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LearnPA5-87983
antibody from Invitrogen Antibodies
Targeting: ABCA4
ABCR, ARMD2, CORD3, FFM, RP19, STGD, STGD1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-87983 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ABCA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: U-251MG, BT-474, 293T, Mouse heart, Mouse lung, Rat eye; Cellular Location: Membrane, Multi-pass membrane protein Immunogen sequence: QELLMNAPES QHLGRIWTEL HILSQFMDTL RTHPERIAGR GIRIRDILKD EETLTLFLIK NIGLSDSVVY LLINSQVRPE QFAHGVPDLA LKDIACSEAL LERFIIFSQR RGAKTVRYAL CSLSQGTLQW IEDTLYANVD FFKLFRVLPT LLDSRSQGIN LRSWGGILSD MSPRIQEFIH RPSMQDLLWV T
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.56 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Molecular Analysis of the ABCA4 Gene Mutations in Patients with Stargardt Disease Using Human Hair Follicles.
Ścieżyńska A, Soszyńska M, Komorowski M, Podgórska A, Krześniak N, Nogowska A, Smolińska M, Szulborski K, Szaflik JP, Noszczyk B, Ołdak M, Malejczyk J
International journal of molecular sciences 2020 May 13;21(10)
International journal of molecular sciences 2020 May 13;21(10)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using ABCA4 Polyclonal antibody (Product # PA5-87983) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using ABCA4 Polyclonal antibody (Product # PA5-87983) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Expression of the ABCA4 gene in hair follicles, skin cells and skin lysates measured at the protein level with Western blot assay. HF, hair follicle; Skin, skin sample positive for ABCA4 mRNA; K, keratinocytes; F, fibroblasts; M, melanocytes; C(+), retinoblastoma lysate; C(-), skin sample negative for ABCA4 mRNA; Std, broad-range protein standard.
