20566-1-AP

antibody from Proteintech Group

Targeting: DDX58 DKFZp434J1111, FLJ13599, RIG-1, RIG-I, RIG1

Provider product page for 20566-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [49]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 20566-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20566-1-AP, RRID:AB_10700006
Product name: RIG-1/DDX58 antibody
Antibody type: Polyclonal
Description: KD/KO validated RIG-1/DDX58 antibody (Cat. #20566-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IP, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

DDX58 protein structure - 20566-1-AP shown in red.

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Zhou Y, Li T, Zhang Y, Zhang N, Guo Y, Gao X, Peng W, Shu S, Zhao C, Cui D, Sun H, Sun Y, Liu J, Tang J, Zhang R, Pu J
PLoS pathogens 2024 Mar;20(3):e1012110

NR4A1 depletion inhibits colorectal cancer progression by promoting necroptosis via the RIG-I-like receptor pathway.

Zhu J, Li J, Yang K, Chen Y, Wang J, He Y, Shen K, Wang K, Shi T, Chen W
Cancer letters 2024 Mar 31;585:216693

REXO2 up-regulation is positively correlated with poor prognosis and tumor immune infiltration in hepatocellular carcinoma.

Zhang T, Zhang R, Zhang Z, Li D, Guo X, Zhang Z, Zhu X, Tan S
International immunopharmacology 2024 Mar 30;130:111740

Hyperacetylated microtubules assist porcine deltacoronavirus nsp8 to degrade MDA5 via SQSTM1/p62-dependent selective autophagy.

Li Z, Lai Y, Qiu R, Tang W, Ren J, Xiao S, Fang P, Fang L
Journal of virology 2024 Mar 19;98(3):e0000324

Mammalian orthoreovirus capsid protein σ3 antagonizes RLR-mediated antiviral responses by degrading MAVS.

Li D, Mo R, Li X, Cheng R, Xie J, Li H, Yang Y, Li S, Li H, Yan Z, Wei S, Idris A, Li X, Feng R
mSphere 2024 Jun 25;9(6):e0023624

Comparison of Neuroinflammation Induced by Hyperphosphorylated Tau Protein Versus Ab42 in Alzheimer's Disease.

Rice M, Nuovo GJ, Sawant D, Mishra A, Tili E
Molecular neurobiology 2024 Jul;61(7):4589-4601

miR-26a exerts broad-spectrum antiviral effects via the enhancement of RIG-I-mediated type I interferon response by targeting USP15.

Zhang J, Li C, Hou Y, Liu D, Li Q, Wang Z, Tang R, Zheng K, Guo H, Wang W
Microbiology spectrum 2024 Jan 11;12(1):e0312423

PI3K/AKT mediated De novo fatty acid synthesis regulates RIG-1/MDA-5-dependent type I IFN responses in BVDV-infected CD8(+)T cells.

Liu SS, Bai TT, Que TL, Luo A, Liang YX, Song YX, Liu TY, Chen JW, Li J, Li N, Zhang ZC, Chen NN, Liu Y, Zhang ZC, Zhou YL, Wang X, Zhu ZB
Veterinary microbiology 2024 Apr;291:110034

Therapeutic efficacy of a novel self-assembled immunostimulatory siRNA combining apoptosis promotion with RIG-I activation in gliomas.

Chen J, Liu Z, Fang H, Su Q, Fan Y, Song L, He S
Journal of translational medicine 2024 Apr 29;22(1):395

IFNα-induced BST2(+) tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.

Zheng C, Wang J, Zhou Y, Duan Y, Zheng R, Xie Y, Wei X, Wu J, Shen H, Ye M, Kong B, Liu Y, Xu P, Zhang Q, Liang T
Cell reports 2024 Apr 23;43(4):114088

Ste20-Like Kinase TAOK1 Positively Regulates Antiviral Responses by Controlling the TBK1-IRF3 Signaling Axis.

Luo X, Ji R, Liu Q, Xiao X, Song W, An H, Li Y, Zhou J
Journal of innate immunity 2023;15(1):380-396

Label-free quantitative proteomic analyses of mouse astrocytes provides insight into the host response mechanism at different developmental stages of Toxoplasma gondii.

Xie H, Sun H, Dong H, Dai L, Xu H, Zhang L, Wang Q, Zhang J, Zhao G, Xu C, Yin K
PLoS neglected tropical diseases 2023 Sep;17(9):e0011102

Type I/type III IFN and related factors regulate JEV infection and BBB endothelial integrity.

Zhang YG, Zhang HX, Chen HW, Lv P, Su J, Chen YR, Fu ZF, Cui M
Journal of neuroinflammation 2023 Sep 27;20(1):216

RIG-I promotes cell proliferation in esophageal squamous cell carcinoma by facilitating p21 degradation.

Wang M, Zhang Y, Gao L, Zhang H, Yang Z, Liu J, Shan W, Zeng L, Zhang R, Li Y, Liu J
Medical oncology (Northwood, London, England) 2023 Sep 1;40(10):288

Naringenin Improves Innate Immune Suppression after PRRSV Infection by Reactivating the RIG-I-MAVS Signaling Pathway, Promoting the Production of IFN-I.

Yu J, Shi H, Song K, Yang Y, Li X, Peng L, Fu B, Yi P
Viruses 2023 Oct 29;15(11)

Capsaicin functions as a selective degrader of STAT3 to enhance host resistance to viral infection.

Zhang MQ, Jia X, Cheng CQ, Wang YX, Li YY, Kong LD, Li QQ, Xie F, Yu YL, He YT, Dong QT, Jia ZH, Wang Y, Xu AL
Acta pharmacologica Sinica 2023 Nov;44(11):2253-2264

Porcine promyelocytic leukemia protein isoforms suppress Japanese encephalitis virus replication in PK15 cells.

Chen Z, Liu H, Zhu J, Duan X, Wang H, Li X, Zhou X, Zhao A, Yang S
Virology journal 2023 Nov 29;20(1):280

TRIM28 negatively regulates the RLR signaling pathway by targeting MAVS for degradation via K48-linked polyubiquitination.

Chen YY, Ran XH, Ni RZ, Mu D
The Journal of biological chemistry 2023 May;299(5):104660

Prediction and Verification of Curcumin as a Potential Drug for Inhibition of PDCoV Replication in LLC-PK1 Cells.

Wang X, Wang X, Zhang J, Shan Q, Zhu Y, Xu C, Wang J
International journal of molecular sciences 2023 Mar 20;24(6)

Cryptosporidium uses CSpV1 to activate host type I interferon and attenuate antiparasitic defenses.

Deng S, He W, Gong AY, Li M, Wang Y, Xia Z, Zhang XT, Huang Pacheco AS, Naqib A, Jenkins M, Swanson PC, Drescher KM, Strauss-Soukup JK, Belshan M, Chen XM
Nature communications 2023 Mar 16;14(1):1456

The m6A Reader YTHDF2 Promotes Bladder Cancer Progression by Suppressing RIG-I-Mediated Immune Response.

Zhang L, Li Y, Zhou L, Zhou H, Ye L, Ou T, Hong H, Zheng S, Zhou Z, Wu K, Yan Z, Thiery JP, Cui J, Wu S
Cancer research 2023 Jun 2;83(11):1834-1850

The differential expression of toll like receptors and RIG-1 in the placenta of neonates with in utero infections.

Rice M, Nicol A, Nuovo GJ
Annals of diagnostic pathology 2023 Feb;62:152080

Heterogeneous Nuclear Protein U Degraded the m(6)A Methylated TRAF3 Transcript by YTHDF2 To Promote Porcine Epidemic Diarrhea Virus Replication.

Zhou H, Yan Y, Gao J, Ma M, Liu Y, Shi X, Zhang Q, Xu X
Journal of virology 2023 Feb 28;97(2):e0175122

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion.

Zheng W, Zhou Z, Rui Y, Ye R, Xia F, Guo F, Liu X, Su J, Lou M, Yu XF
Signal transduction and targeted therapy 2023 Feb 24;8(1):79

An HBV susceptibility variant of KNG1 modulates the therapeutic effects of interferons α and λ1 in HBV infection by promoting MAVS lysosomal degradation.

Zhang B, Han H, Zhao X, Li AN, Wang Y, Yuan W, Yang Z, Li MD
EBioMedicine 2023 Aug;94:104694

The differential expression of toll like receptors and RIG-1 correlates to the severity of infectious diseases.

Rice M, Tili E, Loghmani H, Nuovo GJ
Annals of diagnostic pathology 2023 Apr;63:152102

ARIH1 inhibits influenza A virus replication and facilitates RIG-I dependent immune signaling by interacting with SQSTM1/p62.

Wang S, Li Z, Chen Y, Gao S, Qiao J, Liu H, Song H, Ao D, Sun X
Virology journal 2023 Apr 1;20(1):58

Activation of DDX58/RIG‑I suppresses the growth of tumor cells by inhibiting STAT3/CSE signaling in colon cancer.

Deng Y, Fu H, Han X, Li Y, Zhao W, Zhao X, Yu C, Guo W, Lei K, Wang T
International journal of oncology 2022 Oct;61(4)

Peste des Petits Ruminants Virus Upregulates STING to Activate ATF6-Mediated Autophagy.

Zhang R, Lin H, You Q, Zhang Z, Bai L, Chen F, Zhu X, Zhang Z, Li Y
Journal of virology 2022 Oct 26;96(20):e0137522

Inhibition of LSD1 with Bomedemstat Sensitizes Small Cell Lung Cancer to Immune Checkpoint Blockade and T-Cell Killing.

Hiatt JB, Sandborg H, Garrison SM, Arnold HU, Liao SY, Norton JP, Friesen TJ, Wu F, Sutherland KD, Rienhoff HY, Martins R, Houghton AM, Srivastava S, MacPherson D
Clinical cancer research : an official journal of the American Association for Cancer Research 2022 Oct 14;28(20):4551-4564

Zika virus causes placental pyroptosis and associated adverse fetal outcomes by activating GSDME.

Zhao Z, Li Q, Ashraf U, Yang M, Zhu W, Gu J, Chen Z, Gu C, Si Y, Cao S, Ye J
eLife 2022 Aug 16;11

STING1 is essential for an RNA-virus triggered autophagy.

Zhang R, Qin X, Yang Y, Zhu X, Zhao S, Zhang Z, Su Q, Zhao Z, Yin X, Meng X, Zhang Z, Li Y
Autophagy 2022 Apr;18(4):816-828

Tumor Necrosis Factor-α-Induced Protein 8-Like 2 Negatively Regulates Innate Immunity Against RNA Virus by Targeting RIG-I in Macrophages.

Zou Z, Li M, Zhou Y, Li J, Pan T, Lai L, Wang Q, Zhang L, Wang Q, Song Y, Zhang Y
Frontiers in immunology 2021;12:642715

METTL3 regulates viral m6A RNA modification and host cell innate immune responses during SARS-CoV-2 infection.

Li N, Hui H, Bray B, Gonzalez GM, Zeller M, Anderson KG, Knight R, Smith D, Wang Y, Carlin AF, Rana TM
Cell reports 2021 May 11;35(6):109091

Riok3 inhibits the antiviral immune response by facilitating TRIM40-mediated RIG-I and MDA5 degradation.

Shen Y, Tang K, Chen D, Hong M, Sun F, Wang S, Ke Y, Wu T, Sun R, Qian J, Du Y
Cell reports 2021 Jun 22;35(12):109272

IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.

Jiang Z, Wei F, Zhang Y, Wang T, Gao W, Yu S, Sun H, Pu J, Sun Y, Wang M, Tong Q, Gao C, Chang KC, Liu J
Nature microbiology 2021 Jul;6(7):932-945

The natural compound Cirsitakaoside enhances antiviral innate responses against vesicular stomatitis virus in vitro and in vivo.

Di Q, Zhu H, Pu D, Zhao X, Li X, Ma X, Xiao W, Chen W
International immunopharmacology 2020 Sep;86:106783

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Wang C, Li Y, Yan S, Wang H, Shao X, Xiao M, Yang B, Qin G, Kong R, Chen R, Zhang N
Nature communications 2020 Jun 22;11(1):3162

Butyrate Reprograms Expression of Specific Interferon-Stimulated Genes.

Chemudupati M, Kenney AD, Smith AC, Fillinger RJ, Zhang L, Zani A, Liu SL, Anderson MZ, Sharma A, Yount JS
Journal of virology 2020 Jul 30;94(16)

RIG-I aggravates interstitial fibrosis via c-Myc-mediated fibroblast activation in UUO mice.

Zhou Z, Ni J, Li J, Huo C, Miao N, Yin F, Cheng Q, Xu D, Xie H, Chen P, Zheng P, Zhang Y, Zhou L, Zhang W, Yu C, Liu J, Lu L
Journal of molecular medicine (Berlin, Germany) 2020 Apr;98(4):527-540

The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses.

Wang K, Ma H, Liu H, Ye W, Li Z, Cheng L, Zhang L, Lei Y, Shen L, Zhang F
Cell reports 2019 May 14;27(7):2075-2091.e5

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

Ringeard M, Marchand V, Decroly E, Motorin Y, Bennasser Y
Nature 2019 Jan;565(7740):500-504

Ribosome biogenesis restricts innate immune responses to virus infection and DNA.

Bianco C, Mohr I
eLife 2019 Dec 16;8

Influenza Virus NS1 Protein-RNA Interactome Reveals Intron Targeting.

Zhang L, Wang J, Muñoz-Moreno R, Kim M, Sakthivel R, Mo W, Shao D, Anantharaman A, García-Sastre A, Conrad NK, Fontoura BMA
Journal of virology 2018 Dec 15;92(24)

Defining the Role of Stress Granules in Innate Immune Suppression by the Herpes Simplex Virus 1 Endoribonuclease VHS.

Burgess HM, Mohr I
Journal of virology 2018 Aug 1;92(15)

Activation of the RLR/MAVS Signaling Pathway by the L Protein of Mopeia Virus.

Zhang LK, Xin QL, Zhu SL, Wan WW, Wang W, Xiao G
Journal of virology 2016 Nov 15;90(22):10259-10270

The ORF3 Protein of Genotype 1 Hepatitis E Virus Suppresses TLR3-induced NF-κB Signaling via TRADD and RIP1.

He M, Wang M, Huang Y, Peng W, Zheng Z, Xia N, Xu J, Tian D
Scientific reports 2016 Jun 8;6:27597

The Membrane Protein of Severe Acute Respiratory Syndrome Coronavirus Functions as a Novel Cytosolic Pathogen-Associated Molecular Pattern To Promote Beta Interferon Induction via a Toll-Like-Receptor-Related TRAF3-Independent Mechanism.

Wang Y, Liu L
mBio 2016 Feb 9;7(1):e01872-15

Human respiratory syncytial virus infection is inhibited by IFN-induced transmembrane proteins.

Zhang W, Zhang L, Zan Y, Du N, Yang Y, Tien P
The Journal of general virology 2015 Jan;96(Pt 1):170-182

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 20566-1-AP(DDX58 antibody) at dilution of 1:300
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using DDX58 antibody 20566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line