Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 10-4102 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-RIG-I Antibody
- Antibody type
- Monoclonal
- Description
- RIG-I or Probable ATP-dependent RNA helicase DDX58 or simply DDX58 is a RNA helicase enzyme with two isoforms (106 and 101 kDa). RIG-I belongs to the DEAD/H box family and is involved in several cellular responses including RNA binding and alteration of RNA secondary structure. RIG-1 acts as sensor of viral nucleic acids thus activating a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. Generally it remains as a monomer but upon activation by viral dsRNA it homomultimerizes and interacts with mitochondria antiviral signaling protein (MAVS/IPS1) which activating a cascade of reactions to produce specific interferon. RIG-1 is mostly expressed in vascular smooth cells.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant human
RIG-I protein (amino acids 1-220) w
as used as the immunogen for this a
ntibody.- Isotype
- IgG
- Antibody clone number
- ABM40B5
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
No comments: Submit comment
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of RIG-I. Anti- RIG-I antibody (Clone: ABM40B5) was used at 2 µg/ml on K562 lysates.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RIG-I in human prostate tissue using RIG-I antibody (Clone: ABM40B5) at 5 µg/ml.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 µg/10^6 cells of Anti-RIGI antibody (ABM40B5). Green represent isotype control and red represent Anti-RIG I antibody (10-4102 Abeomics). Goat anti-mouse PE conjugate was used as secondary.
- Protocol
- Protocol