16060-1-AP

antibody from Invitrogen Antibodies

Targeting: NMD3 CGI-07

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16060-1-AP

Provider

Invitrogen Antibodies

Product name

NMD3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: KAVIQVRQK TLHKKTFYYL EQLILKYGMH QNTLRIKEIH DGLDFYYSSK QHAQKMVEFL QCTVPCRYKA SQRLISQDIH SNTYNYKSTF SVEIVPICKD NVVCLSPKLA QSLGNMNQIC VCIRVTSAIH LIDPNTLQVA DIDGSTFWSH PFNSLCHPKQ LEEFIVMECS IVQDIKRAAG AGMISKKHTL GEVWVQKTSE MNTDKQYFCR THLGHLLNPG DLVLGFDLAN CNLNDEHVNK MNSDRVPDVV LIKKSYDRTK RQRRRNWKLK ELARERENMD TDDERQYQDF LEDLEEDEAI RKNVNIYRDS AIPVESDTDD EGAPRISLAE MLEDLHISQD ATGEEGASML T (154-503 aa encoded by BC013317)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 16060-1-AP (NMD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 16060-1-AP (NMD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 16060-1-AP (NMD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 16060-1-AP (NMD3 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 16060-1-AP (NMD3 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 16060-1-AP ( NMD3 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 16060-1-AP ( NMD3 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NMD3 (IP:16060-1-AP, 3ug; Detection:16060-1-AP 1:800) with HepG2 cells lysate 1720ug.