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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB4298 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4298, RRID:AB_1570979
- Product name
- AGL polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of AGL.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A role for AGL ubiquitination in the glycogen storage disorders of Lafora and Cori's disease.
Cheng A, Zhang M, Gentry MS, Worby CA, Dixon JE, Saltiel AR
Genes & development 2007 Oct 1;21(19):2399-409
Genes & development 2007 Oct 1;21(19):2399-409
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using AGL polyclonal antibody (Cat # PAB4298) at 1 : 500 dilution . A total of 20 ug of lysates was loaded for each tissue . Data courtesy of Dr . Alan Cheng, Department of Internal Medicine, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, Michigan .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Expression of myc-GS causes wild type but not the CBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL CBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.
- Validation comment
- Immunofluorescence
