BS5918

antibody from Bioworld Technology, Inc

Targeting: RTCA RPC, RTC1, RTCD1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

BS5918

Provider

Bioworld Technology, Inc

Product name

RTCD1 polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide, corresponding to amino acids 321-366 of Human RTCD1.

Description

RTCD1 catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. There are two named isoforms.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Bioworld Technology, Inc (provider)

Main image

Experimental details

Western blot (WB) analysis of RTCD1 pAb at 1:500 dilutionLane1:Hela cell lysateLane2:NIH-3T3 cell lysateLane3:H9C2 cell lysate