Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 12828-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EXTL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LPSVKEDKM LMLRREIKSQ GKSTMDSFTL IMQTYNRTDL LLKLLNHYQA VPNLHKVIVV WNNIGEKAPD ELWNSLGPHP IPVIFKQQTA NRMRNRLQVF PELETNAVLM VDDDTLISTP DLVFAFSVWQ QFPDQIVGFV PRKHVSTSSG IYSYGSFEMQ APGSGNGDQY SMVLIGASFF NSKYLELFQR QPAAVHALID DTQNCDDIAM NFIIAKHIGK TSGIFVKPVN MDNLEKETNS GYSGMWHRAE HALQRSYCIN KLVNIYDSMP LRYSNITISQ FGFPYANYKR KI (40-330 aa encoded by BC036015)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12828-1-AP (EXTL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 12828-1-AP (EXTL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 12828-1-AP (EXTL2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 12828-1-AP (EXTL2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).