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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-C397231 - Provider product page
- Provider
- LSBio
- Product name
- CTP Synthetase 2 / CTPS2 Antibody LS-C397231
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: CTPS2 antibody at 2.8µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating CTPS2 in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CTPS2 Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CTPS2 Antibody (6µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating CTPS2 in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of CTPS2 Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CTPS2 Antibody (6µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (20µg)
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunocytochemistry analysis of human gastric cancer using CTPS2 Antibody at dilution of 1:100
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunocytochemistry analysis of human prostate tissue using CTPS2 Antibody at dilution of 1:100
