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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-77312 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-77312, RRID:AB_1085329
- Product name
- Rabbit Polyclonal PHC3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHC3 Antibody [NB100-77312] - Detection of Human PHC3 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PHC3 antibody NB100-77312 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PHC3 was also immunoprecipitated by another rabbit anti-PHC3 antibody, which recognizes a downstream epitope.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHC3 Antibody [NB100-77312] - Detection of human PHC3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHC3 antibody NB100-77312 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PHC3 Antibody [NB100-77312] - Detection of human PHC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHC3 antibody NB100-77312 used for IP at 3 ug per reaction. PHC3 was also immunoprecipitated by rabbit anti-PHC3 antibody NB100-77313. For blotting immunoprecipitated PHC3, NB100-77312 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
