PA5-47588

antibody from Invitrogen Antibodies

Targeting: ITGB6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47588

Provider

Invitrogen Antibodies

Product name

ITGB6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse Integrin beta 6 is observed and less than 5% cross-reactivity with recombinant human (rh) Integrin beta 3 and rhIntegrin beta 5 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Reactivity

Human, Mouse

Host

Sheep

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-ITGB6 Polyclonal Antibody (Product # PA5-47588) and bands between 86-110 kDa corresponding to ITGB6 were observed across the cell lines and tissue tested. HT-29, MCF 10A, A-431 were high expressing cell lines when compared to A549 and HEK-293; Mouse Kidney, Mouse Lung were high expressing tissues when compared to Mouse Liver and Mouse Ovary. Membrane enriched extracts (30 µg lysate) of HT-29 (Lane 1), SK-BR-3 (Lane 2), A549 (Lane 3), MCF 10A (Lane 4), A-431 (Lane 5), HEK-293 (Lane 6), tissue extracts of Mouse Kidney (Lane 7), Mouse Lung (Lane 8), Mouse Liver (Lane 9) and Mouse Ovary (Lane 10)were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.1µg/mL) and detected by chemiluminescence with Rabbit anti-Sheep IgG (H+L) Secondary Antibody, HRP (Product # 61-8620, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of ITGB6 in immersion fixed HT‚29 human colon adenocarcinoma cell line. Samples were incubated in ITGB6 polyclonal antibody (Product # PA5-47588) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of ITGB6 in immersion fixed HT‚29 human colon adenocarcinoma cell line. Samples were incubated in ITGB6 polyclonal antibody (Product # PA5-47588) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Integrin ß6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Sheep Anti-human Integrin ß6 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47588) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Sheep IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HT-29 human colon adenocarcinoma cell line was stained with Sheep Anti-human Integrin β6 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47588, filled histogram) or isotype control antibody (open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of ITGB6 in HT‚29 human colon adenocarcinoma cell line. Samples were incubated in ITGB6 polyclonal antibody (Product # PA5-47588) isotype control antibody followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody.