TA807408

antibody from OriGene

Targeting: ECT2 ARHGEF31

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA807408

Provider

OriGene

Proper citation

OriGene Cat#TA807408, RRID:AB_2628435

Product name

ECT2 mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)

Antibody type

Monoclonal

Description

ECT2 mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)

Host

Mouse

Conjugate

Unconjugated

Epitope

ECT2

Isotype

IgG

Antibody clone number

OTI2D9

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECT2 (RC211319, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECT2.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 1 cell line by using anti-ECT2 monoclonal antibody.(1:200)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ECT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807408)

Validation comment

IHC