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Western blot
ELISA
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunoprecipitation [2]
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Validation data
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- Product number
- LS-C673888 - Provider product page
- Provider
- LSBio
- Product name
- ECT2 Antibody LS-C673888
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate All lanes:ECT2 antibody at 2.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 104, 100, 109, 101 kDa Observed band size: 104 kDa
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate All lanes:ECT2 antibody at 2.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 104, 100, 109, 101 kDa Observed band size: 104 kDa
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating ECT2 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of ECT2 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000) Lane 2: ECT2 Antibody (6µg) + Hela whole cell lysate (1mg) Lane 3: Hela whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating ECT2 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of ECT2 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000) Lane 2: ECT2 Antibody (6µg) + Hela whole cell lysate (1mg) Lane 3: Hela whole cell lysate (20µg)
