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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-113489 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ECT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 1 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ECT2 using a polyclonal antibody (Product # PA5-113489) at a dilution of 2 µg/mL. A goat anti-rabbit polyclonal secondary antibody at a dilution of 1:50,000 was used for detection in Hela whole cell lysate, A549 whole cell lysate, and 293T whole cell lysate. Observed band: 104 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of ECT2 in Hela whole cell lysate using a Polyclonal antibody (Product #PA5-113489). Samples were treated with 6 µg of the primary antibody and an HRP-conjugated Protein G secondary antibody was used at a dilution of 1:50,000. Lane 1: Rabbit control IgG, Lane 2: 1 mg of Hela whole cell lysate, Lane 3: 20 µg of Hela whole cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded ECT2 in human kidney tissue using a ECT2 polyclonal antibody (Product # PA5-113489) at a dilution of 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). The section was blocked with 10% normal goat serum for 30 min at room temperature. The primary antibody, in 1% BSA, was incubated at 4°C overnight. A biotinylated secondary antibody was used with an HRP conjugated SP system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of ECT2 in Hela whole cell lysate using a Polyclonal antibody (Product #PA5-113489). Samples were treated with 6 µg of the primary antibody and an HRP-conjugated Protein G secondary antibody was used at a dilution of 1:50,000. Lane 1: Rabbit control IgG, Lane 2: 1 mg of Hela whole cell lysate, Lane 3: 20 µg of Hela whole cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of ECT2 in Hela whole cell lysate using a Polyclonal antibody (Product #PA5-113489). Samples were treated with 6 µg of the primary antibody and an HRP-conjugated Protein G secondary antibody was used at a dilution of 1:50,000. Lane 1: Rabbit control IgG, Lane 2: 1 mg of Hela whole cell lysate, Lane 3: 20 µg of Hela whole cell lysate.
