PAB22425

antibody from Abnova Corporation

Targeting: AKNAD1 C1orf62, MGC26989

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22425

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22425, RRID:AB_10968137

Product name

C1orf62 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant C1orf62.

Antigen sequence

LEWKQNVEKKGHGRINCGRFSIVLHEKAPHSDSTP
NSDTGHSFYSDSGTEMQSNKCQDCGTKIPTSRRAC
RKEPTKEFHY


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with C1orf62 polyclonal antibody (Cat # PAB22425) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with C1orf62 polyclonal antibody (Cat # PAB22425) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node with C1orf62 polyclonal antibody (Cat # PAB22425) shows moderate cytoplasmic positivity in reactions center cells and in a fraction of cells outside the reaction center at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)