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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-38042 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MFN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A recommended positive control is A431 cell lysate.
- Concentration
- 1 mg/mL
Submitted references Low expression of mitofusin 1 is associated with mitochondrial dysfunction and apoptosis in porcine somatic cell nuclear transfer embryos.
Intracardiac administration of ephrinA1-Fc preserves mitochondrial bioenergetics during acute ischemia/reperfusion injury.
Park MR, Hwang IS, Kwak TU, Lim JH, Hwang S, Cho SK
Animal science journal = Nihon chikusan Gakkaiho 2020 Jan-Dec;91(1):e13430
Animal science journal = Nihon chikusan Gakkaiho 2020 Jan-Dec;91(1):e13430
Intracardiac administration of ephrinA1-Fc preserves mitochondrial bioenergetics during acute ischemia/reperfusion injury.
Torres MJ, McLaughlin KL, Renegar RH, Valsaraj S, Whitehurst KS, Sharaf OM, Sharma UM, Horton JL, Sarathy B, Parks JC, Brault JJ, Fisher-Wellman KH, Neufer PD, Virag JAI
Life sciences 2019 Dec 15;239:117053
Life sciences 2019 Dec 15;239:117053
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MFN1 in A431 cell lysate using a MFN1 polyclonal antibody (Product # PA5-38042) at a dilution of 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MFN1 in A431 cell lysate with MFN1 Polyclonal Antibody (Product # PA5-38042) at 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of MFN1 in A431 cells using a MFN1 polyclonal antibody (Product # PA5-38042) at a dilution of 5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of MFN1 in A431 cells with MFN1 Polyclonal Antibody (Product # PA5-38042) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 2 Figure Expression level of MFN1 in porcine PA and SCNT blastocysts using immunofluorescence staining and RT-qPCR. (A) (upper panels) PA blastocysts, (lower panels) SCNT blastocysts. Fluorescence images of blastocysts showing MFN1 expression levels. DAPI (A,a), MFN1 (B,b), and Merged (C,c). Scale bar = 100 mum. (B) Quantification of MFN1 fluorescence signal shown in part A. (C) Quantification of MFN1 mRNA levels by RT-qPCR. Plotted are the M +- SD . * p < .05 versus corresponding SCNT value. PA, parthenogenetic activation; SCNT, somatic cell nuclear transfer; MFN1, mitofusin 1; DAPI, 4',6-diamidino-2-phenylindole
