H00055669-M04

antibody from Novus Biologicals

Targeting: MFN1 FLJ20693

Provider product page for H00055669-M04

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Flow cytometry

Blocking/Neutralizing

Antibody data

Antibody Data
Antigen structure
References [26]
Comments [0]
Validations
Western blot [4]
ELISA [1]

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Product number: H00055669-M04 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#H00055669-M04, RRID:AB_2142623
Product name: Mouse Monoclonal Mitofusin 1 Antibody
Antibody type: Monoclonal
Description: IgG purified. MFN1 - mitofusin 1. This antibody may cross react with MFN2 protein.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Vial size: 0.1 mg
Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

MFN1 protein structure - H00055669-M04 shown in red.

Submitted references ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects.

Zhao Y, Sun X, Hu D, Prosdocimo DA, Hoppel C, Jain MK, Ramachandran R, Qi X
Nature communications 2019 Mar 26;10(1):1371

A selective inhibitor of mitofusin 1-βIIPKC association improves heart failure outcome in rats.

Ferreira JCB, Campos JC, Qvit N, Qi X, Bozi LHM, Bechara LRG, Lima VM, Queliconi BB, Disatnik MH, Dourado PMM, Kowaltowski AJ, Mochly-Rosen D
Nature communications 2019 Jan 18;10(1):329

Acquired Expression of Mutant Mitofusin 2 Causes Progressive Neurodegeneration and Abnormal Behavior.

Ishikawa K, Yamamoto S, Hattori S, Nishimura N, Tani H, Mito T, Matsumoto H, Miyakawa T, Nakada K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Feb 27;39(9):1588-1604

Inhibiting neddylation modification alters mitochondrial morphology and reprograms energy metabolism in cancer cells.

Zhou Q, Li H, Li Y, Tan M, Fan S, Cao C, Meng F, Zhu L, Zhao L, Guan MX, Jin H, Sun Y
JCI insight 2019 Feb 21;4(4)

NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.

Furuya N, Kakuta S, Sumiyoshi K, Ando M, Nonaka R, Suzuki A, Kazuno S, Saiki S, Hattori N
EMBO reports 2018 Dec;19(12)

MicroRNA-668 represses MTP18 to preserve mitochondrial dynamics in ischemic acute kidney injury.

Wei Q, Sun H, Song S, Liu Y, Liu P, Livingston MJ, Wang J, Liang M, Mi QS, Huo Y, Nahman NS, Mei C, Dong Z
The Journal of clinical investigation 2018 Dec 3;128(12):5448-5464

Sirtuin 5 protects mitochondria from fragmentation and degradation during starvation.

Guedouari H, Daigle T, Scorrano L, Hebert-Chatelain E
Biochimica et biophysica acta. Molecular cell research 2017 Jan;1864(1):169-176

Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.

Vukotic M, Nolte H, König T, Saita S, Ananjew M, Krüger M, Tatsuta T, Langer T
Molecular cell 2017 Aug 3;67(3):471-483.e7

Mitophagy receptor FUNDC1 regulates mitochondrial dynamics and mitophagy.

Chen M, Chen Z, Wang Y, Tan Z, Zhu C, Li Y, Han Z, Chen L, Gao R, Liu L, Chen Q
Autophagy 2016;12(4):689-702

Lysine 63-linked polyubiquitination is dispensable for Parkin-mediated mitophagy.

Shiba-Fukushima K, Inoshita T, Hattori N, Imai Y
The Journal of biological chemistry 2014 Nov 28;289(48):33131-6

A small natural molecule promotes mitochondrial fusion through inhibition of the deubiquitinase USP30.

Yue W, Chen Z, Liu H, Yan C, Chen M, Feng D, Yan C, Wu H, Du L, Wang Y, Liu J, Huang X, Xia L, Liu L, Wang X, Jin H, Wang J, Song Z, Hao X, Chen Q
Cell research 2014 Apr;24(4):482-96

PARK2/Parkin-mediated mitochondrial clearance contributes to proteasome activation during slow-twitch muscle atrophy via NFE2L1 nuclear translocation.

Furuya N, Ikeda S, Sato S, Soma S, Ezaki J, Oliva Trejo JA, Takeda-Ezaki M, Fujimura T, Arikawa-Hirasawa E, Tada N, Komatsu M, Tanaka K, Kominami E, Hattori N, Ueno T
Autophagy 2014 Apr;10(4):631-41

Phosphatidic acid (PA)-preferring phospholipase A1 regulates mitochondrial dynamics.

Baba T, Kashiwagi Y, Arimitsu N, Kogure T, Edo A, Maruyama T, Nakao K, Nakanishi H, Kinoshita M, Frohman MA, Yamamoto A, Tani K
The Journal of biological chemistry 2014 Apr 18;289(16):11497-511

Atad3 function is essential for early post-implantation development in the mouse.

Goller T, Seibold UK, Kremmer E, Voos W, Kolanus W
PloS one 2013;8(1):e54799

Regulation of miRNAs in human skeletal muscle following acute endurance exercise and short-term endurance training.

Russell AP, Lamon S, Boon H, Wada S, Güller I, Brown EL, Chibalin AV, Zierath JR, Snow RJ, Stepto N, Wadley GD, Akimoto T
The Journal of physiology 2013 Sep 15;591(18):4637-53

Dynamics of nucleoid structure regulated by mitochondrial fission contributes to cristae reformation and release of cytochrome c.

Ban-Ishihara R, Ishihara T, Sasaki N, Mihara K, Ishihara N
Proceedings of the National Academy of Sciences of the United States of America 2013 Jul 16;110(29):11863-8

Suppressor of cytokine signaling 6 (SOCS6) promotes mitochondrial fission via regulating DRP1 translocation.

Lin HY, Lai RH, Lin ST, Lin RC, Wang MJ, Lin CC, Lee HC, Wang FF, Chen JY
Cell death and differentiation 2013 Jan;20(1):139-53

Extramitochondrial OPA1 and adrenocortical function.

Fülöp L, Rajki A, Katona D, Szanda G, Spät A
Molecular and cellular endocrinology 2013 Dec 5;381(1-2):70-9

TRAP1 controls mitochondrial fusion/fission balance through Drp1 and Mff expression.

Takamura H, Koyama Y, Matsuzaki S, Yamada K, Hattori T, Miyata S, Takemoto K, Tohyama M, Katayama T
PloS one 2012;7(12):e51912

PINK1-mediated phosphorylation of the Parkin ubiquitin-like domain primes mitochondrial translocation of Parkin and regulates mitophagy.

Shiba-Fukushima K, Imai Y, Yoshida S, Ishihama Y, Kanao T, Sato S, Hattori N
Scientific reports 2012;2:1002

Apoptosis inducing factor deficiency causes reduced mitofusion 1 expression and patterned Purkinje cell degeneration.

Chung SH, Calafiore M, Plane JM, Pleasure DE, Deng W
Neurobiology of disease 2011 Feb;41(2):445-57

Rab32 modulates apoptosis onset and mitochondria-associated membrane (MAM) properties.

Bui M, Gilady SY, Fitzsimmons RE, Benson MD, Lynes EM, Gesson K, Alto NM, Strack S, Scott JD, Simmen T
The Journal of biological chemistry 2010 Oct 8;285(41):31590-602

Impaired balance of mitochondrial fission and fusion in Alzheimer's disease.

Wang X, Su B, Lee HG, Li X, Perry G, Smith MA, Zhu X
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Jul 15;29(28):9090-103

Effects of overexpression of huntingtin proteins on mitochondrial integrity.

Wang H, Lim PJ, Karbowski M, Monteiro MJ
Human molecular genetics 2009 Feb 15;18(4):737-52

PGC-1alpha's relationship with skeletal muscle palmitate oxidation is not present with obesity despite maintained PGC-1alpha and PGC-1beta protein.

Holloway GP, Perry CG, Thrush AB, Heigenhauser GJ, Dyck DJ, Bonen A, Spriet LL
American journal of physiology. Endocrinology and metabolism 2008 Jun;294(6):E1060-9

OPA1 mutations associated with dominant optic atrophy impair oxidative phosphorylation and mitochondrial fusion.

Zanna C, Ghelli A, Porcelli AM, Karbowski M, Youle RJ, Schimpf S, Wissinger B, Pinti M, Cossarizza A, Vidoni S, Valentino ML, Rugolo M, Carelli V
Brain : a journal of neurology 2008 Feb;131(Pt 2):352-67

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - MFN1 monoclonal antibody (M04), clone 3C9 Western Blot analysis of MFN1 expression in HeLa ( Cat # L013V1 ).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Western blot analysis of MFN1 over-expressed 293 cell line, cotransfected with MFN1 Validated Chimera RNAi or non-transfected control. Blot probed with H00055669-M04. GAPDH (36.1 kDa) used as loading control.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Analysis of MFN1 expression in transfected 293T cell line by MFN1 monoclonal antibody (M04), clone 3C9. Lane 1: MFN1 transfected lysatE (84.2 KDa). Lane 2: Non-transfected lysate.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Analysis of MFN1 expression in HepG2.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: ELISA: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Detection limit for recombinant GST tagged MFN1 is 0.3 ng/ml as a capture antibody.