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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- F52859 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ACTL6A Antibody / BAF53A
- Antibody type
- Polyclonal (Antigen purified)
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse, Rat, Bovine, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Storage
- Aliquot the BAF53A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) mouse NIH3T3, and 3) mouse skeletal muscle lysate with BAF53A antibody at 1:1000. Expected molecular weight: 47-53 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent image of human HeLa cells stained with BAF53A antibody diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
