AP15957PU-T

antibody from Acris Antibodies GmbH

Targeting: PYCARD ASC, CARD5, TMS-1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP15957PU-T

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP15957PU-T, RRID:AB_10977729

Product name

anti CARD5 / PYCARD / ASC (C-term)

Antibody type

Polyclonal

Antigen

Peptide from C Terminus of the protein sequence according to NP_037390.2; NP_660183.1

Reactivity

Human

Host

Goat

Vial size

20 µg

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP15957PU-N (1 µg/ml) staining of HeLa lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

HEK293 overexpressing ASC (RC215592) and probed with AP15957PU-N (mock transfection in first lane).

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP15957PU-N (2.5 µg/ml) staining of Paraffin Embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.