STJ72990

antibody from St John's Laboratory

Targeting: PYCARD ASC, CARD5, TMS-1

Western blot

ELISA

Antibody data

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Validation data

Product number: STJ72990 - Provider product page
Provider: St John's Laboratory
Product name: Anti-Pycard antibody (Internal) (STJ72990)
Antibody type: Polyclonal
Description: Goat polyclonal antibody anti-Pycard (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Reactivity: Human, Mouse, Rat, Bovine, Canine, Porcine
Host: Goat
Conjugate: Unconjugated
Antigen sequence: DLTDKLVSYYLES
Epitope: NA
Isotype: IgG
Antibody clone number: NA
Vial size: NA
Concentration: NA
Storage: Store at-20 on receipt and minimise freeze-thaw cycles.
Handling: NA

PYCARD protein structure - STJ72990 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: STJ72990 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: STJ72990 Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: STJ72990 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA