PA5-95826

antibody from Invitrogen Antibodies

Targeting: PYCARD ASC, CARD5, TMS-1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-95826

Provider

Invitrogen Antibodies

Product name

PYCARD Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: HT-29, BT-474, U-937, mouse thymus, mouse spleen, rat spleen; Cellular Location: Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus Immunogen sequence: MGRARDAILD ALENLTAEEL KKFKLKLLSV PLREGYGRIP RGALLSMDAL DLTDKLVSFY LETYGAELTA NVLRDMGLQE MAGQLQAATH Q

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.65 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Colchicine Ameliorates Dilated Cardiomyopathy Via SIRT2-Mediated Suppression of NLRP3 Inflammasome Activation.

Sun X, Duan J, Gong C, Feng Y, Hu J, Gu R, Xu B
Journal of the American Heart Association 2022 Jul 5;11(13):e025266

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PYCARD was performed in U-2 OS cells using PYCARD Polyclonal Antibody (Product # PA5-95826).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PYCARD was performed in U-2 OS cells using PYCARD Polyclonal Antibody (Product # PA5-95826).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PYCARD in paraffin-embedded human tonsil. Samples were incubated with PYCARD Polyclonal antibody (Product # PA5-95826) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PYCARD in paraffin-embedded human esophageal cancer. Samples were incubated with PYCARD Polyclonal antibody (Product # PA5-95826) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of PYCARD was performed in 200 µg extracts of U937 cells using PYCARD Polyclonal Antibody (Product # PA5-95826). Western blot was performed from the immunoprecipitate using PYCARD Polyclonal Antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 NLRP3 (NOD-like receptor protein 3) inflammasome pathway was inhibited by colchicine treatment. A and B , Representative immunoblots and the corresponding analysis of NLRP3 inflammasome pathway in cardiac tissues at the end point of experiment. GAPDH shows as loading control. C , Representative immunofluorescence images of cardiac tissues staining with ASC (green), cTNT (cardiac troponin T) (red), and DAPI (blue). Frequency of ASC speck is shown on the right (n=6-8 each group). Five images per mouse are evaluated. Scale bar=50 mum. Data are shown as mean+-SEM. All data statistical significance are determined by 1-way ANOVA (Tukey post-test). cTNT indicates cardiac troponin T; DCM, dilated cardiomyopathy; IL-1beta, interleukin-1beta; and NLRP3, NOD-like receptor protein 3. * P