Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A00362-4 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-TMS1/ASC/Pycard Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for TMS1/ASC/PYCARD detection. Tested with WB, IHC-P, FCM, Direct ELISA in Mouse;Rat.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Phoenixin 20 ameliorates pulmonary arterial hypertension via inhibiting inflammation and oxidative stress.
Vaccarin alleviates septic cardiomyopathy by potentiating NLRP3 palmitoylation and inactivation.
Carnosine ameliorates postoperative cognitive dysfunction of aged rats by limiting astrocytes pyroptosis.
Chai Y, Gu X, Zhang H, Xu X, Chen L
Aging 2024 Mar 19;16(6):5027-5037
Aging 2024 Mar 19;16(6):5027-5037
Vaccarin alleviates septic cardiomyopathy by potentiating NLRP3 palmitoylation and inactivation.
Zhu XX, Meng XY, Zhang AY, Zhao CY, Chang C, Chen TX, Huang YB, Xu JP, Fu X, Cai WW, Hou B, Du B, Zheng GL, Zhang JR, Lu QB, Bai N, Han ZJ, Bao N, Qiu LY, Sun HJ
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Aug;131:155771
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Aug;131:155771
Carnosine ameliorates postoperative cognitive dysfunction of aged rats by limiting astrocytes pyroptosis.
Shen J, Xu J, Wen Y, Tang Z, Li J, Sun J
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2024 Apr 25;21(4):e00359
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2024 Apr 25;21(4):e00359
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of TMS1/ASC/PYCARD using anti-TMS1/ASC/PYCARD antibody (A00362-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMS1/ASC/PYCARD antigen affinity purified polyclonal antibody (Catalog # A00362-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMS1/ASC/PYCARD at approximately 22KD. The expected band size for TMS1/ASC/PYCARD is at 22KD
- Additional image