NBP1-78977

antibody from Novus Biologicals

Targeting: PYCARD ASC, CARD5, TMS-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

NBP1-78977

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-78977, RRID:AB_11015255

Product name

Rabbit Polyclonal ASC/TMS1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Blockade of β-adrenergic signaling suppresses inflammasome and alleviates cardiac fibrosis.

Dang S, Zhang ZY, Li KL, Zheng J, Qian LL, Liu XY, Wu Y, Zhang CY, Zhao XX, Yu ZM, Wang RX, Jiang T
Annals of translational medicine 2020 Feb;8(4):127

---

Anti-inflammatory effect of omega unsaturated fatty acids and dialysable leucocyte extracts on collagen-induced arthritis in DBA/1 mice.

Pérez-Martínez PI, Rojas-Espinosa O, Hernández-Chávez VG, Arce-Paredes P, Estrada-Parra S
International journal of experimental pathology 2020 Feb;101(1-2):55-64

---

Involvement of NLRP3 and NLRC4 Inflammasome in Uropathogenic E. coli Mediated Urinary Tract Infections.

Verma V, Gupta S, Kumar P, Yadav S, Dhanda RS, Gaind R, Arora R, Frimodt-Møller N, Yadav M
Frontiers in microbiology 2019;10:2020

---

Evaluation of Canonical Inflammasome Activation in Human Monocytes by Imaging Flow Cytometry.

Lage SL, Dominical VM, Wong CS, Sereti I
Frontiers in immunology 2019;10:1284

---

NLRP3 inflammasome activation in platelets in response to sepsis.

Cornelius DC, Baik CH, Travis OK, White DL, Young CM, Austin Pierce W, Shields CA, Poudel B, Williams JM
Physiological reports 2019 May;7(9):e14073

---

PM2.5 exposure impairs sperm quality through testicular damage dependent on NALP3 inflammasome and miR-183/96/182 cluster targeting FOXO1 in mouse.

Zhou L, Su X, Li B, Chu C, Sun H, Zhang N, Han B, Li C, Zou B, Niu Y, Zhang R
Ecotoxicology and environmental safety 2019 Mar;169:551-563

---

Oral Administration of Compound Probiotics Ameliorates HFD-Induced Gut Microbe Dysbiosis and Chronic Metabolic Inflammation via the G Protein-Coupled Receptor 43 in Non-alcoholic Fatty Liver Disease Rats.

Liang Y, Liang S, Zhang Y, Deng Y, He Y, Chen Y, Liu C, Lin C, Yang Q
Probiotics and antimicrobial proteins 2019 Mar;11(1):175-185

---

AIM2 gene silencing attenuates diabetic cardiomyopathy in type 2 diabetic rat model.

Wang X, Pan J, Liu H, Zhang M, Liu D, Lu L, Tian J, Liu M, Jin T, An F
Life sciences 2019 Mar 15;221:249-258

---

Silybin inhibits NLRP3 inflammasome assembly through the NAD⁺/SIRT2 pathway in mice with nonalcoholic fatty liver disease.

Zhang B, Xu D, She L, Wang Z, Yang N, Sun R, Zhang Y, Yan C, Wei Q, Aa J, Liu B, Wang G, Xie Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Feb;32(2):757-767

---

S. aureus Evades Macrophage Killing through NLRP3-Dependent Effects on Mitochondrial Trafficking.

Cohen TS, Boland ML, Boland BB, Takahashi V, Tovchigrechko A, Lee Y, Wilde AD, Mazaitis MJ, Jones-Nelson O, Tkaczyk C, Raja R, Stover CK, Sellman BR
Cell reports 2018 Feb 27;22(9):2431-2441

---

NLRP3 inflammasome expression in lipopolysaccharide-induced otitis media.

Kariya S, Okano M, Zhao P, Maeda Y, Kataoka Y, Higaki T, Noda Y, Makihara S, Nishizaki K
Acta oto-laryngologica 2018 Dec;138(12):1061-1065

---

Actin polymerization plays a significant role in asbestos-induced inflammasome activation in mesothelial cells in vitro.

MacPherson M, Westbom C, Kogan H, Shukla A
Histochemistry and cell biology 2017 May;147(5):595-604

---

Activation of NLRP3 inflammasome in human middle ear cholesteatoma and chronic otitis media.

Kariya S, Okano M, Zhao P, Kataoka Y, Yoshinobu J, Maeda Y, Ishihara H, Higaki T, Nishizaki K
Acta oto-laryngologica 2016;136(2):136-40

---

Corosolic acid ameliorates acute inflammation through inhibition of IRAK-1 phosphorylation in macrophages.

Kim SJ, Cha JY, Kang HS, Lee JH, Lee JY, Park JH, Bae JH, Song DK, Im SS
BMB reports 2016 May;49(5):276-81

---

Corosolic acid ameliorates acute inflammation through inhibition of IRAK-1 phosphorylation in macrophages.

Kim SJ, Cha JY, Kang HS, Lee JH, Lee JY, Park JH, Bae JH, Song DK, Im SS
BMB reports 2016 May;49(5):276-81

---

Mitochondrial ROS Induces Cardiac Inflammation via a Pathway through mtDNA Damage in a Pneumonia-Related Sepsis Model.

Yao X, Carlson D, Sun Y, Ma L, Wolf SE, Minei JP, Zang QS
PloS one 2015;10(10):e0139416

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: ASC/TMS1 Antibody [NBP1-78977] - Lane view shows a specific band for ASC TMS1 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ASC/TMS1 Antibody [NBP1-78977] - Whole cell protein from (1) THP1 and (2) HL60 was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-ACS/TMS1 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78977] - Tested in mouse intestine at a 1:400 dilution.