RQ6434

antibody from NSJ Bioreagents

Targeting: PYCARD ASC, CARD5, TMS-1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ6434

Provider

NSJ Bioreagents

Product name

Pycard Antibody / Tms1 / mAsc

Antibody type

Polyclonal

Description

This highly specific Pycard antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Direct ELISA applications with mouse and rat samples.

Reactivity

Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Pycard antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat lung, 2) rat spleen, 3) rat thymus, 4) mouse lung, 5) mouse spleen and 6) mouse thymus tissue lysate with Pycard antibody. Predicted molecular weight ~22 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse intestine tissue with Pycard antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse RAW264.7 cells with Pycard antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Pycard antibody.