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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- AM26452AF-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti CARD5 / PYCARD / ASC
- Antibody type
- Monoclonal
- Antigen
- Triton X-100 insoluble fraction of retinoic acid-treated HL-60 cells
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 23-4
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of ASC expression in U937 (1), HL-60 (2), Jurkat(3), WR19L(4) and PC12 (5) using AM26452AF-N.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitationof ASC from HL-60 with AM26452AF-NÂ (1, 3) or normal mouse IgG (2, 4). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26452AF-NÂ (1, 2) or normal mouse IgG (3, 4).
