AF3715

antibody from Novus Biologicals

Targeting: FAP DPPIV

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3715

Provider

Novus Biologicals

Product name

Sheep Polyclonal Fibroblast Activation Protein alpha/FAP Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Fibroblast Activation Protein alpha/FAP in direct ELISAs and Western blots.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Highly versatile cancer photoimmunotherapy using photosensitizer-conjugated avidin and biotin-conjugated targeting antibodies.

Shirasu N, Shibaguchi H, Yamada H, Kuroki M, Yasunaga S
Cancer cell international 2019;19:299

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Fibroblast activation protein-positive fibroblasts promote tumor progression through secretion of CCL2 and interleukin-6 in esophageal squamous cell carcinoma.

Higashino N, Koma YI, Hosono M, Takase N, Okamoto M, Kodaira H, Nishio M, Shigeoka M, Kakeji Y, Yokozaki H
Laboratory investigation; a journal of technical methods and pathology 2019 Jun;99(6):777-792

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Patch repair of deep wounds by mobilized fascia.

Correa-Gallegos D, Jiang D, Christ S, Ramesh P, Ye H, Wannemacher J, Kalgudde Gopal S, Yu Q, Aichler M, Walch A, Mirastschijski U, Volz T, Rinkevich Y
Nature 2019 Dec;576(7786):287-292

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Tumor-targeting efficacy of a BF211 prodrug through hydrolysis by fibroblast activation protein-α.

Chai XP, Sun GL, Fang YF, Hu LH, Liu X, Zhang XW
Acta pharmacologica Sinica 2018 Mar;39(3):415-424

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Cancer-associated fibroblasts induce antigen-specific deletion of CD8 (+) T Cells to protect tumour cells.

Lakins MA, Ghorani E, Munir H, Martins CP, Shields JD
Nature communications 2018 Mar 5;9(1):948

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A synthetic urinary probe-coated nanoparticles sensitive to fibroblast activation protein α for solid tumor diagnosis.

Feng X, Wang Q, Liao Y, Zhou X, Wang Y, Liu W, Zhang G
International journal of nanomedicine 2017;12:5359-5372

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Fibroblast activation protein augments progression and metastasis of pancreatic ductal adenocarcinoma.

Lo A, Li CP, Buza EL, Blomberg R, Govindaraju P, Avery D, Monslow J, Hsiao M, Puré E
JCI insight 2017 Oct 5;2(19)

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Adrenergic-mediated increases in INHBA drive CAF phenotype and collagens.

Nagaraja AS, Dood RL, Armaiz-Pena G, Kang Y, Wu SY, Allen JK, Jennings NB, Mangala LS, Pradeep S, Lyons Y, Haemmerle M, Gharpure KM, Sadaoui NC, Rodriguez-Aguayo C, Ivan C, Wang Y, Baggerly K, Ram P, Lopez-Berestein G, Liu J, Mok SC, Cohen L, Lutgendorf SK, Cole SW, Sood AK
JCI insight 2017 Aug 17;2(16)

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Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice.

Fan MH, Zhu Q, Li HH, Ra HJ, Majumdar S, Gulick DL, Jerome JA, Madsen DH, Christofidou-Solomidou M, Speicher DW, Bachovchin WW, Feghali-Bostwick C, Puré E
The Journal of biological chemistry 2016 Apr 8;291(15):8070-89

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Antitumor effects of chimeric receptor engineered human T cells directed to tumor stroma.

Kakarla S, Chow KK, Mata M, Shaffer DR, Song XT, Wu MF, Liu H, Wang LL, Rowley DR, Pfizenmaier K, Gottschalk S
Molecular therapy : the journal of the American Society of Gene Therapy 2013 Aug;21(8):1611-20

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Fibroblast Activation Protein alpha/FAP by Western Blot. Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein alpha/FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fibroblast Activation Protein alpha/FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Fibroblast Activation Protein alpha/FAP in Human Squamous Cell Carcinoma. Fibroblast Activation Protein alpha/FAP was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein alpha/FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Fibroblast Activation Protein alpha/FAP in Human Basal Cell Carcinoma. Fibroblast Activation Protein alpha/FAP was detected in immersion fixed paraffin-embedded sections of human basal cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein alpha/FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.