Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00002191-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002191-M01, RRID:AB_538711
- Product name
- Mouse Monoclonal Fibroblast Activation Protein alpha/FAP Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. FAP - fibroblast activation protein, alpha
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Cancer-associated fibroblasts (CAFs) promote the lymph node metastasis of esophageal squamous cell carcinoma.
Fibroblast activation protein (FAP) is essential for the migration of bone marrow mesenchymal stem cells through RhoA activation.
Extracellular Hsp90 mediates an NF-κB dependent inflammatory stromal program: implications for the prostate tumor microenvironment.
Fibroblast activation protein and chronic liver disease.
Fibroblast activation protein and chronic liver disease.
Differential impact of TGF-beta and EGF on fibroblast differentiation and invasion reciprocally promotes colon cancer cell invasion.
Kashima H, Noma K, Ohara T, Kato T, Katsura Y, Komoto S, Sato H, Katsube R, Ninomiya T, Tazawa H, Shirakawa Y, Fujiwara T
International journal of cancer 2019 Feb 15;144(4):828-840
International journal of cancer 2019 Feb 15;144(4):828-840
Fibroblast activation protein (FAP) is essential for the migration of bone marrow mesenchymal stem cells through RhoA activation.
Chung KM, Hsu SC, Chu YR, Lin MY, Jiaang WT, Chen RH, Chen X
PloS one 2014;9(2):e88772
PloS one 2014;9(2):e88772
Extracellular Hsp90 mediates an NF-κB dependent inflammatory stromal program: implications for the prostate tumor microenvironment.
Bohonowych JE, Hance MW, Nolan KD, Defee M, Parsons CH, Isaacs JS
The Prostate 2014 Apr;74(4):395-407
The Prostate 2014 Apr;74(4):395-407
Fibroblast activation protein and chronic liver disease.
Wang XM, Yao TW, Nadvi NA, Osborne B, McCaughan GW, Gorrell MD
Frontiers in bioscience : a journal and virtual library 2008 Jan 1;13:3168-80
Frontiers in bioscience : a journal and virtual library 2008 Jan 1;13:3168-80
Fibroblast activation protein and chronic liver disease.
Wang XM, Yao TW, Nadvi NA, Osborne B, McCaughan GW, Gorrell MD
Frontiers in bioscience : a journal and virtual library 2008 Jan 1;13:3168-80
Frontiers in bioscience : a journal and virtual library 2008 Jan 1;13:3168-80
Differential impact of TGF-beta and EGF on fibroblast differentiation and invasion reciprocally promotes colon cancer cell invasion.
Denys H, Derycke L, Hendrix A, Westbroek W, Gheldof A, Narine K, Pauwels P, Gespach C, Bracke M, De Wever O
Cancer letters 2008 Aug 8;266(2):263-74
Cancer letters 2008 Aug 8;266(2):263-74
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Analysis of FAP expression in transfected 293T cell line by FAP monoclonal antibody (M01), clone 1E5.Lane 1: FAP transfected lysate(87.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - FAP monoclonal antibody (M01), clone 1E5 Analysis of FAP expression in HepG2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Fibroblast Activation Protein alpha/FAP Antibody (1E5.) [H00002191-M01] - Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Detection limit for recombinant GST tagged FAP is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Analysis of FAP transfected lysate using anti-FAP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FAP MaxPab rabbit polyclonal antibody.