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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10209 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10209, RRID:AB_1674727
- Product name
- Fap polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Fap.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Abrogation of fibroblast activation protein enzymatic activity attenuates tumor growth.
FAPalpha, a surface peptidase expressed during wound healing, is a tumor suppressor.
Expression of the fibroblast activation protein during mouse embryo development.
Cheng JD, Valianou M, Canutescu AA, Jaffe EK, Lee HO, Wang H, Lai JH, Bachovchin WW, Weiner LM
Molecular cancer therapeutics 2005 Mar;4(3):351-60
Molecular cancer therapeutics 2005 Mar;4(3):351-60
FAPalpha, a surface peptidase expressed during wound healing, is a tumor suppressor.
Ramirez-Montagut T, Blachere NE, Sviderskaya EV, Bennett DC, Rettig WJ, Garin-Chesa P, Houghton AN
Oncogene 2004 Jul 15;23(32):5435-46
Oncogene 2004 Jul 15;23(32):5435-46
Expression of the fibroblast activation protein during mouse embryo development.
Niedermeyer J, Garin-Chesa P, Kriz M, Hilberg F, Mueller E, Bamberger U, Rettig WJ, Schnapp A
The International journal of developmental biology 2001 Apr;45(2):445-7
The International journal of developmental biology 2001 Apr;45(2):445-7
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using Fap polyclonal antibody (Cat # PAB10209) shows detection of Fap protein in whole cell lysates from Fap expressing HEK cells (Lane 4), but not control HEK cells (Lane 3).Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively).The band at ~90 KDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP.Primary antibody was used at 1:1,000.Personal communication, S.Kim, NCI, Bethesda, MD.
