Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BAF3715 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Fibroblast Activation Protein alpha/FAP Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Fibroblast Activation Protein alpha/FAP in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human DPPIV is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Biotin
- Antigen sequence
Q12884
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Fibroblast activation protein augments progression and metastasis of pancreatic ductal adenocarcinoma.
Cancer-Associated Fibroblasts in Pancreatic Cancer Are Reprogrammed by Tumor-Induced Alterations in Genomic DNA Methylation.
Lo A, Li CP, Buza EL, Blomberg R, Govindaraju P, Avery D, Monslow J, Hsiao M, Puré E
JCI insight 2017 Oct 5;2(19)
JCI insight 2017 Oct 5;2(19)
Cancer-Associated Fibroblasts in Pancreatic Cancer Are Reprogrammed by Tumor-Induced Alterations in Genomic DNA Methylation.
Xiao Q, Zhou D, Rucki AA, Williams J, Zhou J, Mo G, Murphy A, Fujiwara K, Kleponis J, Salman B, Wolfgang CL, Anders RA, Zheng S, Jaffee EM, Zheng L
Cancer research 2016 Sep 15;76(18):5395-404
Cancer research 2016 Sep 15;76(18):5395-404
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Fibroblast Activation Protein alpha/FAP by Western Blot. Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein alpha/FAP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3715) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for Fibroblast Activation Protein alpha/FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.