H00025801-M04

antibody from Abnova Corporation

Targeting: GCA GCL

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00025801-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00025801-M04, RRID:AB_10551569

Product name

GCA monoclonal antibody (M04), clone 2F11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant GCA.

Antigen sequence

MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILL
DGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDA
EELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDH
TGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVE
HHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFF
DDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDF
LQGTMAI


Isotype

IgG

Antibody clone number

2F11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

GCA monoclonal antibody (M04), clone 2F11. Western Blot analysis of GCA expression in IMR-32(Cat # L008V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GCA is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol