RQ4647

antibody from NSJ Bioreagents

Targeting: GCA GCL

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4647

Provider

NSJ Bioreagents

Product name

Grancalcin Antibody / GCA

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Storage

After reconstitution, the Grancalcin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) placenta, 2) HL-60 and 3) Caco-2 lysate with Grancalcin antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) placenta, 2) HL-60 and 3) Caco-2 lysate with Grancalcin antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human MCF7, 2) human HL-60, 3) human HeLa, 4) rat testis and 5) mouse testis tissue lysate with Grancalcin antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human HeLa cells with Grancalcin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human bladder urothelial carcinoma tissue with Grancalcin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colorectal adenocarcinoma tissue with Grancalcin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung cancer tissue with Grancalcin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HL60 cells with Grancalcin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Grancalcin antibody.