Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [7]
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Validation data
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- Product number
- NBP1-89785 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89785, RRID:AB_11022545
- Product name
- Rabbit Polyclonal Grancalcin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Grancalcin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Grancalcin Antibody [NBP1-89785] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Grancalcin Antibody [NBP1-89785] - Analysis using Anti-GCA antibody NBP1-89785 (A) shows similar pattern to independent antibody NBP1-89786 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89785] - Staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89785] - Staining of human bone marrow shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89785] - Staining of human cerebral cortex shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89785] - Staining in human bone marrow and cerebral cortex tissues using anti-GCA antibody. Corresponding GCA RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89785] - Staining of human liver.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89785] - Staining of human bone marrow, cerebral cortex, kidney and liver using Anti-GCA antibody NBP1-89785 (A) shows similar protein distribution across tissues to independent antibody NBP1-89786 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Grancalcin Antibody [NBP1-89785] - Staining of human kidney.