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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-78606 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSRC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, A431, human PSRC1-transfected 293T Predicted Reactivity: Rhesus Monkey (95%), Bovine (86%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.86 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PSRC1 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with PSRC1 polyclonal antibody (Product # PA5-78606) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PSRC1 in A) 293T whole cell lysate, B) whole cell lysate of human PSRC1-transfected 293T cells using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with PSRC1 polyclonal antibody (Product # PA5-78606) using a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PSRC1 Polyclonal Antibody detects PSRC1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PSRC1 Polyclonal Antibody (Product # PA5-78606) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PSRC1 Polyclonal Antibody detects PSRC1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human PSRC1-transfected 293T cells.12 % SDS-PAGE. PSRC1 Polyclonal Antibody (Product # PA5-78606) dilution: 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PSRC1 Polyclonal Antibody detects PSRC1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PSRC1 protein stained by PSRC1 Polyclonal Antibody (Product # PA5-78606) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PSRC1 was performed in paraffin-embedded mouse brain tissue using PSRC1 Polyclonal Antibody (Product # PA5-78606) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of PSRC1 was performed in frozen-sectioned mouse mouse cerebellum tissue using PSRC1 Polyclonal Antibody (Product # PA5-78606) at a dilution of 1:250 (Green). Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
