LS-C312938

antibody from LSBio

Targeting: HSPE1 CPN10, EPF, GroES, HSP10

Antibody data

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Validation data

Product number: LS-C312938 - Provider product page
Provider: LSBio
Product name: HSPE1 / HSP10 / Chaperonin 10 Antibody (aa81-102) LS-C312938
Antibody type: Polyclonal
Description: Immunogen affinity purified
Reactivity: Human, Rat, Bovine, Goat, Horse, Porcine, Rabbit, Sheep, Simian
Host: Rabbit
Storage: At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

HSPE1 protein structure - LS-C312938 shown in red.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: WB of HSPE1 / HSP10 / Chaperonin 10 antibody. Lane 1: Rat Thymus Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: Rat Ovary Tissue Lysate. Lane 4: Rat Testis Tissue Lysate. Lane 5: A431 Cell Lysate. Lane 6: A549 Cell Lysate. Lane 7: MCF-7 Cell Lysate. Lane 8: MM231 Cell Lysate. Lane 9: HELA Cell Lysate.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis of Cpn10 using anti-Cpn10 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Thymus Tissue Lysate Lane 2: Rat Brain Tissue Lysate Lane 3: Rat Ovary Tissue Lysate Lane 4: Rat Testis Tissue Lysate Lane 5: A431 Whole Cell Lysate Lane 6: A549 Whole Cell Lysate Lane 7: MCF-7 Whole Cell Lysate Lane 8: MM231 Whole Cell Lysate Lane 9: HELA Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cpn10 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cpn10 at approximately 11 KD. The expected band size for Cpn10 is at 11 KD.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: HSPE1 / HSP10 / Chaperonin 10 antibody. IHC(P): Human Intestinal Cancer Tissue.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: HSPE1 / HSP10 / Chaperonin 10 antibody. IHC(P): Mouse Intestine Tissue.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC analysis of Cpn10 using anti-Cpn10 antibody. Cpn10 was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Cpn10 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: HSPE1 / HSP10 / Chaperonin 10 antibody. IHC(P): Human Intestinal Cancer Tissue.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC analysis of Cpn10 using anti-Cpn10 antibody. Cpn10 was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Cpn10 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.