Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF3298 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal HSP10/EPF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human and mouse HSP10/EPF in Western blots.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Circulating heat shock protein 60 levels are elevated in HIV patients and are reduced by anti-retroviral therapy.
Anraku I, Rajasuriar R, Dobbin C, Brown R, Lewin SR, Suhrbier A
PloS one 2012;7(9):e45291
PloS one 2012;7(9):e45291
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse HSP10/EPF by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, A431 human epithelial carcinoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3298) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for HSP10/EPF was detected at approximately ??? kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 4.