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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- PA5-22340 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GNAT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (87%), Xenopus laevis (91%), Chicken (94%), Bovine (99%).
- Concentration
- 1.26 mg/mL
Submitted references BBSome Component BBS5 Is Required for Cone Photoreceptor Protein Trafficking and Outer Segment Maintenance.
Bales KL, Bentley MR, Croyle MJ, Kesterson RA, Yoder BK, Gross AK
Investigative ophthalmology & visual science 2020 Aug 3;61(10):17
Investigative ophthalmology & visual science 2020 Aug 3;61(10):17
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GNAT2 using Whole cell extracts (30 µg). Samples were loaded onto a 10% SDS-PAGE gel and probed with a GNAT2 polyclonal antibody (Product # PA5-22340) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GNAT2 using 30 µg of A549 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GNAT2 polyclonal antibody (Product # PA5-22340) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-GNAT2 Rabbit Polyclonal Antibody (Product # PA5-22340) and a 40kDa band corresponding to GNAT2 was observed in Mouse Retina and was absent in Hep G2, HEK-293, A549, COLO 205, Mouse Spleen, Mouse Brain and Mouse Heart which are reported negative for GNAT2 expression. Membrane enriched cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (lane 2), A549 (Lane 3), COLO 205 (Lane 4), Mouse Retina (Lane 5), Mouse Spleen (Lane 6) Mouse Brain (Lane 7) and Mouse Heart (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of GNAT2 was performed in frozen sectioned adult mouse retina tissue using GNAT2 Polyclonal Antibody (Product # PA5-22340) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of GNAT2 was performed in frozen sectioned adult mouse retina tissue using GNAT2 Polyclonal Antibody (Product # PA5-22340) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.
