ASC-002-25UL
antibody from Invitrogen Antibodies
Targeting: SCN2A
HBSCI, HBSCII, Nav1.2, SCN2A1, SCN2A2
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- ASC-002-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCN2A (NaV1.2) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof rat brain membranes: - 1. Anti-SCN2A (NaV1.2) Antibody (#ASC-002), (1:200). 2. Anti-SCN2A (NaV1.2) Antibody , preincubated with SCN2A/Nav1.2 Blocking Peptide (#BLP-SC002).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof rat brain membranes: - 1. Anti-SCN2A (NaV1.2) Antibody (#ASC-002), (1:200). 2. Anti-SCN2A (NaV1.2) Antibody , preincubated with SCN2A/Nav1.2 Blocking Peptide (#BLP-SC002).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of NaV1.2 in mouse hippocampus - Immunohistochemical staining of mouse hippocampus using Anti-SCN2A (NaV1.2) Antibody (#ASC-002 ). A. NaV1.2 (red) is present in dendrites of pyramidal neurons in the CA3 region. B. Staining ofinterneurons in the pyramidal layer with mouse Anti-Parvalbumin (green) demonstrates the restriction of NaV1.2 to dendrites (arrows) extending from the pyramidal layer (P). C. Confocal merge of panels A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of rat brain lysate: - 1. Rat brain lysates2. Lysates immunoprecipitated with Anti-SCN2A (NaV1.2) Antibody (#ASC-002), (6 µg).3. Lysates immunoprecipitated with pre-immune rabbit serum. The lower arrow indicates the IgG heavy chain. Western blot analysiswas performed with Anti-SCN2A (NaV1.2) Antibody .