PA1-007-A488

antibody from Invitrogen Antibodies

Targeting: PDIA4 ERP70, ERP72

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-007-A488

Provider

Invitrogen Antibodies

Product name

ERp72 Polyclonal Antibody, Alexa Fluor™ 488

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1007A488 detects ERp72 protein in human and mouse samples. The PA1007A488 immunogen is a synthetic peptide corresponding to residues K(629) F I E E H A T K L S R T K E E L(645) of human ERp72. This peptide (Cat. # PEP-223) is available for use in neutralization and control experiments.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Green dye

Isotype

IgG

Vial size

50 µL

Concentration

1 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ERp72 (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a ERp72 Polyclonal Antibody, AlexaFluor 488 conjugate (Product # PA1-007-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Conjugate

Green dye