AP1827A

antibody from Abgent

Targeting: LAMP3 CD208, DC-LAMP, DCLAMP, LAMP, TSC403

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP1827A

Provider

Abgent

Proper citation

Abgent Cat#AP1827a, RRID:AB_1208788

Product name

LAMP3 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

Hypoxic regulation and prognostic value of LAMP3 expression in breast cancer.

Nagelkerke A, Mujcic H, Bussink J, Wouters BG, van Laarhoven HW, Sweep FC, Span PN
Cancer 2011 Aug 15;117(16):3670-81

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

LAMP3 Antibody (N-term) (Cat. #AP1827a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the LAMP3 antibody detected the LAMP3 protein (arrow).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent image of U251 cells stained with LAMP3 (N-term) antibody. U251 cells were treated with Chloroquine (50 ¼M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1827a LAMP3 (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). LAMP3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells."

Primary Ab dilution

1:100