Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- AF4087 - Provider product page
- Provider
- R&D Systems
- Product name
- Human DC-LAMP Lumenal Domain Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human DC-LAMP Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human LAMP is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
EAW78337
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of DC-LAMP in Human Mature Dendritic Cells by Flow Cytometry. Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+ cells treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 7 days and 1 μg/mL LPS, 20 ng/mL Recombinant Human TNF-alpha (Catalog # 210-TA), and 20 ng/mL Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB) for last 24 hours were stained with Goat Anti-Human DC-LAMP Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4087, filled histogram) or isotype control (Catalog # AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Allophycocyanin (Catalog # F0108)