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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- R31035 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HIP Antibody / HSC70 Interacting Protein
- Antibody type
- Polyclonal
- Description
- This highly specific HIP antibody is suitable for use in Western blot/Immunofluorescence applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the HIP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HIP antibody and Lane 1: A431; 2: HeLa; 3: 293T; 4: Jurkat; 5: MCF-7 cell lysate. Expected/observed size ~48KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human 293T, 3) human SK-O-V3, 4) mouse testis and 5) mouse NIH 3T3 cell lysate with HIP antibody. Expected molecular weight: ~48 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human HeLa cells with HIP antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
