MAB3212

antibody from Abnova Corporation

Targeting: ST13 FAM10A1, HIP, HSPABP1, P48, SNC6

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3212

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB3212, RRID:AB_10555885

Product name

ST13 monoclonal antibody, clone AT1F1

Antibody type

Monoclonal

Antigen

Recombinant protein corresponding to full length human ST13.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 μl

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The heat shock protein 70 cochaperone hip enhances functional maturation of glucocorticoid receptor.

Nelson GM, Prapapanich V, Carrigan PE, Roberts PJ, Riggs DL, Smith DF
Molecular endocrinology (Baltimore, Md.) 2004 Jul;18(7):1620-30

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Hop modulates Hsp70/Hsp90 interactions in protein folding.

Johnson BD, Schumacher RJ, Ross ED, Toft DO
The Journal of biological chemistry 1998 Feb 6;273(6):3679-86

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Cell lysates of 293T (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with ST13 monoclonal antibody, clone AT1F1 (Cat # MAB3212 ; 1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Paraffin embedded sections of human gastric cancer tissue were incubated with ST13 monoclonal antibody, clone AT1F1 (Cat # MAB3212 ; 1 : 25) for 2 hours at room temperature.Antigen retrieval was performed in 100 mM sodium citrate buffer and detected using Diaminobenzidine (DAB).

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)