NBP1-48021

antibody from Novus Biologicals

Targeting: STK39 DCHT, SPAK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-48021

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-48021, RRID:AB_10011018

Product name

Mouse Monoclonal STK39 Antibody

Antibody type

Monoclonal

Description

Affinity purified. This antibody is specific for Homo sapiens serine threonine kinase 39 (STE20/SPS1 homolog, yeast) (STK39).

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: STK39 Antibody (4E3) [NBP1-48021] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STK39 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK39.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: STK39 Antibody (4E3) [NBP1-48021] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-STK39 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: STK39 Antibody (4E3) [NBP1-48021] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-STK39 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: STK39 Antibody (4E3) [NBP1-48021] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-STK39 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: STK39 Antibody (4E3) [NBP1-48021] - Staining of paraffin-embedded Human endometrium tissue using anti-STK39 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: STK39 Antibody (4E3) [NBP1-48021] - Staining of paraffin-embedded Human Ovary tissue using anti-STK39 mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: STK39 Antibody (4E3) [NBP1-48021] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-STK39 antibody, and then analyzed by flow cytometry.