PA5-21360

antibody from Invitrogen Antibodies

Targeting: AP2M1 AP50, CLAPM1, mu2

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21360

Provider

Invitrogen Antibodies

Product name

AP2M1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, Raji. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (99%), Dog (100%), Pig (99%), Chicken (97%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.57 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback Loop.

Agajanian MJ, Walker MP, Axtman AD, Ruela-de-Sousa RR, Serafin DS, Rabinowitz AD, Graham DM, Ryan MB, Tamir T, Nakamichi Y, Gammons MV, Bennett JM, Couñago RM, Drewry DH, Elkins JM, Gileadi C, Gileadi O, Godoi PH, Kapadia N, Müller S, Santiago AS, Sorrell FJ, Wells CI, Fedorov O, Willson TM, Zuercher WJ, Major MB
Cell reports 2019 Jan 2;26(1):79-93.e8

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using AP2M1 (Product # PA5-21360) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 WNT Treatment Induces AAK1-, PIP2-Dependent Phosphorylation of AP2M1 (A) Western blot analysis of HT1080 cells treated with WNT3A CM for indicated times. (B) Western blot analysis of HT1080 cells transfected with either AAK1 or control siRNA for 72 hr. Cells were then treated with rhWNT3A (200 ng/mL) for 15 min, then the medium was changed for fresh, complete DMEM, and cells were incubated for 9 hr. (C) Western blot analysis of pAP2M1 levels in HEK293T cells treated with either rhWNT3A (3A) or rhWNT5A (5A) (200 ng/mL) for 9 hr. The box-and-whisker plot represents pAP2M1/AP2M1 expression from four biological replicates quantified by LiCOR software. (D) Western blot analysis of HT1080 cells treated with either CHIR99021 compound (1 muM) or rhWNT3A (200 ng/mL) for the indicated time. (E) HEK293T cells were transfected with FLAG-dnTCF7L2 for 24 hr. Cells were then treated with WNT3A for 15 min, then the medium was changed for fresh, complete DMEM, incubated for 9 hr, and analyzed using western blot. (F) Western blot analysis of HEK293T WT, DVL TKO clone #4, DVL TKO clone #6, and LRP5/6 DKO cells treated with WNT3A CM for 9 hr. (G) Western blot analysis of HT1080 cells treated with WNT3A CM for 15 mins, then the medium was changed for complete DMEM containing either carbachol (50 muM) or neomycin (100 muM). Cells were incubated for 9 hr prior to cell harvest. (H) Western blot analysis of HT1080 cells transfected with siRNA against PI4K2alpha for 72 hr and pulsed with WNT3