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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-20648 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Lass6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is rat brain tissue lysate.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain tissue lysate using a LASS6 polyclonal antibody (Product # PA5-20648) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), MDA-MB-231 (Lane 2), IMR-32 (Lane 3), Jurkat (Lane 4), MOLT-4 (Lane 5) and HT-29 (Lane 6). The blot was probed with Anti-Lass6 Polyclonal Antibody (Product # PA5-20648, 2 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 40 kDa band corresponding to Lass6 was observed across cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LASS6 in rat brain tissue lysate with Lass6 Polyclonal Antibody (Product # PA5-20648) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Lass6 was achieved by transfecting MDA-MB-231 cells with Lass6 specific siRNAs (Silencer® select Product # s12199, s12198). Western blot analysis (Fig. a) was performed using whole cell extracts from the Lass6 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Lass6 Polyclonal Antibody (Product # PA5-20648, 1µg/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Lass6.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of LASS6 in Mouse Brain cells with Lass6 Polyclonal Antibody (Product # PA5-20648) at 5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of LASS6 in Mouse Brain cells with Lass6 Polyclonal Antibody (Product # PA5-20648) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of LASS6 in mouse brain tissue with Lass6 Polyclonal Antibody (Product # PA5-20648) at 2.5 µg/mL.
