PA5-27826

antibody from Invitrogen Antibodies

Targeting: ATP5PB ATP5F1

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-27826 - Provider product page
Provider: Invitrogen Antibodies
Product name: ATP Synthase B1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: Molt-4, mouse brain. Predicted reactivity: Mouse (81%), Rat (80%), Rhesus Monkey (89%), Bovine (88%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.45 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ATP5PB protein structure - PA5-27826 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ATP Synthase B1 Polyclonal Antibody (Product # PA5-27826). Sample (30 µg of whole cell lysate). Lane A: Molt-4. 12% SDS PAGE. ATP Synthase B1 Polyclonal Antibody (Product # PA5-27826) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ATP Synthase B1 Polyclonal Antibody (Product # PA5-27826). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. ATP Synthase B1 Polyclonal Antibody (Product # PA5-27826) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of ATP Synthase B1 was achieved by transfecting HEK-293 cells with ATP Synthase B1 specific siRNAs (Silencer® select Product # s1777, s1779). Western blot analysis (Fig. a) was performed using membrane extracts from the ATP Synthase B1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with ATP Synthase B1 Polyclonal Antibody (Product # PA5-27826, 1:500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ATP Synthase B1. A non specific band (*) was also observed at ~70KDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), NIH/3T3 (Lane 3), Jurkat (Lane 4), Hep G2 (Lane 5), MOLT-4 (Lane 6) and tissue extract (30 µg lysate) of Mouse Brain (Lane 7). The blot was probed with Anti-ATP Synthase B1 Polyclonal Antibody (Product # PA5-27826, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 23 kDa band corresponding to ATP Synthase B1 was detected across the cell lines and tissue tested. An additional band were also observed at ~80kDa in certain cell lines.