Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
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Validation data
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- Product number
- H00005437-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005437-M01, RRID:AB_2252709
- Product name
- Mouse Monoclonal RPB8 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. POLR2H - polymerase (RNA) II (DNA directed) polypeptide H
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Examining the complexity of human RNA polymerase complexes using HaloTag technology coupled to label free quantitative proteomics.
Daniels DL, Méndez J, Mosley AL, Ramisetty SR, Murphy N, Benink H, Wood KV, Urh M, Washburn MP
Journal of proteome research 2012 Feb 3;11(2):564-75
Journal of proteome research 2012 Feb 3;11(2):564-75
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01] - Analysis of POLR2H expression in transfected 293T cell line by POLR2H monoclonal antibody (M01), clone 3G6-1A4.Lane 1: POLR2H transfected lysate(17.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01] - POLR2H monoclonal antibody (M01), clone 3G6-1A4. Analysis of POLR2H expression in HepG2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01] - POLR2H monoclonal antibody (M01), clone 3G6-1A4 Analysis of POLR2H expression in Hela.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RPB8 Antibody (3G6-1A4) [H00005437-M01] - Detection limit for recombinant GST tagged POLR2H is approximately 0.3ng/ml as a capture antibody.