H00005437-M01

antibody from Novus Biologicals

Targeting: POLR2H RPB8

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005437-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00005437-M01, RRID:AB_2252709

Product name

Mouse Monoclonal RPB8 Antibody

Antibody type

Monoclonal

Description

IgG purified. POLR2H - polymerase (RNA) II (DNA directed) polypeptide H

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Examining the complexity of human RNA polymerase complexes using HaloTag technology coupled to label free quantitative proteomics.

Daniels DL, Méndez J, Mosley AL, Ramisetty SR, Murphy N, Benink H, Wood KV, Urh M, Washburn MP
Journal of proteome research 2012 Feb 3;11(2):564-75

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01] - Analysis of POLR2H expression in transfected 293T cell line by POLR2H monoclonal antibody (M01), clone 3G6-1A4.Lane 1: POLR2H transfected lysate(17.1 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01] - POLR2H monoclonal antibody (M01), clone 3G6-1A4. Analysis of POLR2H expression in HepG2.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01] - POLR2H monoclonal antibody (M01), clone 3G6-1A4 Analysis of POLR2H expression in Hela.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Sandwich ELISA: RPB8 Antibody (3G6-1A4) [H00005437-M01] - Detection limit for recombinant GST tagged POLR2H is approximately 0.3ng/ml as a capture antibody.