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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [4]
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- Product number
- NBP1-80816 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-80816, RRID:AB_11022238
- Product name
- Rabbit Polyclonal RPB8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat RPB8 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPB8 Antibody [NBP1-80816] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPB8 Antibody [NBP1-80816] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPB8 Antibody [NBP1-80816] - Analysis using Anti-POLR2H antibody NBP1-80816 (A) shows similar pattern to independent antibody NBP2-76548 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPB8 Antibody [NBP1-80816] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPB8 Antibody [NBP1-80816] - Staining of human stomach shows strong nuclear and cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPB8 Antibody [NBP1-80816] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPB8 Antibody [NBP1-80816] - Staining of human thyroid gland shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPB8 Antibody [NBP1-80816] - Staining in human thyroid gland and pancreas tissues using anti-POLR2H antibody. Corresponding POLR2H RNA-seq data are presented for the same tissues.
