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LearnRQ8257
antibody from NSJ Bioreagents
Targeting: PPM1J
DKFZp434P1514, FLJ35951, MGC19531, PP2Czeta, PPP2CZ
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- RQ8257 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PPM1J Antibody / Protein phosphatase 1J
- Antibody type
- Polyclonal
- Description
- This highly specific PPM1J antibody is suitable for use in Western blot/Immunofluorescence/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PPM1J antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HaCaT, 2) human MCF7, 3) human U-2 OS, 4) human A431, 5) rat brain, 6) rat kidney, 7) mouse brain and 8) mouse kidney tissue lysate with PPM1J antibody. Predicted molecular weight ~55 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with PPM1J antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
