NBP2-36779

antibody from Novus Biologicals

Targeting: GORASP2 GOLPH6, GRASP55, GRS2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-36779

Provider

Novus Biologicals

Product name

Mouse Monoclonal GRASP55 Antibody

Antibody type

Monoclonal

Description

Protein A purified. Specificity of human GRASP55 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Mouse

Antigen sequence

DAASSLTVDVTPPTA


Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GRASP55 Antibody (CL2610) [NBP2-36779] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GRASP55 Antibody (CL2610) [NBP2-36779] - Lane 1: Marker [kDa]. Lane 2: Human cell line Hela cytoplasmic fraction. Lane 3: Human cell line Hela membrane fraction. Lane 4: Human cell line Hela nuclear fraction. Lane 5: Human cell line Hela chromatin fraction. Lane 6: Human cell line Hela cytoskeletal fraction.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GRASP55 Antibody (CL2610) [NBP2-36779] - analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GORASP2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining of human endometrium shows moderate to strong membranous and cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining of human epididymis shows strong positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining of human prostate shows moderate to strong membranous positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: GRASP55 Antibody (CL2610) [NBP2-36779] - Staining in human epididymis and skeletal muscle tissues. Corresponding GORASP2 RNA-seq data are presented for the same tissues.